Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1993-08-10
1995-11-28
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435105, 435200, 4352401, 435243, 43525233, 43525421, 536 232, C12P 1902, C12N 924, C12N 1556
Patent
active
054707250
ABSTRACT:
Novel hybrid thermostable (1,3-1,4)-.beta.-glucanases, their use in food manufacturing and feed manufacturing, DNA fragments encoding such glucanases, organisms expressing the DNA fragments and a method for producing the thermostable (1,3-1,4)-.beta.-glucanases. Hybrid fusion genes encoding Bacillus (1,3-1,4)-.beta.-glucanases were constructed, the gene products of which are more thermostable than any (1,3-1,4)-.beta.-glucanase known until now. The hybrid genes were constructed by reciprocal exchanges of the amino-terminal and carboxy-terminal parts of the .beta.-glucanase encoding genes from Bacillus amyloliquefaciens and Bacillus macerans. The resulting thermostable (1,3-1,4)-.beta.-glucanases retain a significant enzymatic activity at temperatures exceeding 65.degree. C. and at pH values below 5.0.
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Borriss Rainer
Hofemeister Jurgen
Olsen Ole
Thomsen Karl K.
Von Wettstein Dietrich
Akademie der Wissenchaften der DDR
Carlsberg A/S
Patterson Jr. Charles L.
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