Thermophilic phospholipases and method for production thereof

Details Thermophilic phospholipases and method for production thereof Thermophilic phospholipases and method for production thereof

1997-12-01

1999-12-14

Weber, Jon P.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C12N 918

Patent

active

060016264

ABSTRACT:
Disclosed are a thermophilic phospholipase having an optimum temperature range of 95 to 105.degree. C. which is useful in high-temperature degumming processes in oil refining process and high-temperature processing of phospholipids; and a method for producing a thermophilic phospholipase comprising culturing a microorganism capable of producing the phospholipase in a culture medium and collecting the phospholipase from the resultant culture.

REFERENCES:
Adams et al. Chem. Eng. News, vol. 73(50), pp. 32-42, "Enzymes from Microorganisms in Extreme Environments", Dec. 1995.
Sako et al., Int. J. Syst. Bacteriol., vol. 46(4), "Aeropyrum pernix gen. nov., sp. nov., A Novel Aerobic Hyperthermophilic Archaeon Growing at Temperatures Up to 100 Degrees C.", pp. 1070-1077, 1996.
Hartley et al., Biochem. Soc. Symp., vol. 48, "Industrial Prospects for Thermophiles and Thermophilic Enzymes", pp. 133-146, 1983.
Wiegel et al., Crit. Rev. Biotechnol., vol. 3(1), "The Importance of Thermophilic Bacteria in Biotechnology", pp. 39-108, 1986.
Gonzalez et al. Extremophiles, vol. 2(2), "Pyrococcus horikoshii sp. nov., A Hyperthermophilic Archaeon isolated from a Hydrothermal Vent at the Okinawa Trough", pp. 123-130, 1998.
Brennan, M., Chem. Eng. News, vol. 74(42), "Enzyme Discovery Heats Up", pp. 31-33, Oct. 1996.
Aura et al,. Transesterification of Soy Lecithin by Lipase and Phospholipase, J. Am. Oil Chem. Soc. 72 (1995), 1375-1379.
Dahlke et al., First Experiences with Enzymatic Oil Refining, Inform, 6 (1995), 1284-1291.
Kleuser et al., Measurement of Phospholipase A.sub.2 and 1-alkylglycerophosphocholine acetyltransferase Activities in Stimulated Alveolar Macrophages by HPLC Analysis of NBD-Labeled Ether Lipids, Chem. Phys. Lipid 79 (1996), 29-37.
Ohara et al., Protein, Nucleic Acid and Enzyme, 37 (1992), 898-905.
Shen & Cho, Highly Efficient Immobilization of Phospholipase A.sub.2 and its Biomedical Applications, J. Lipid Res. 36 (1995), 1147-1151.
Washburn & Dennis, Novel General Approach for the Assay and Inhibition of Hydrolytic Enzymes Utilizing Suicide-Inhibitory Bifunctionally Linked Substrates (SIBLINKS): Exemplified by a Phospholipase A.sub.2 Assay, J. Am. Chem. Soc. 112 (1990) 2040-2041.

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