Thermophilic collagenases, thermophilic bacteria capable of prod

Details Thermophilic collagenases, thermophilic bacteria capable of prod Thermophilic collagenases, thermophilic bacteria capable of prod

1979-03-30

1982-02-16

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435832, 435253, C12N 954, C12N 120

Patent

active

043159885

ABSTRACT:
This invention relates to a process for producing collagenase, which comprises aerobically cultivating a collagenase-producing bacterium belonging to the genus Bacillus, and recovering collagenase from the culture broth.
This invention also provides a thermophilic bacterium of the genus Bacillus which has the ability to produce collagenase and which grows at a temperature of 42.degree. to 74.degree. C. and a pH of 5.0 to 9.0 and a novel thermophilic collagenase which does not substantially lose collagenase activity even when maintained for 1 hour at a temperature of 60.degree. C. in the presence of a calcium ion at a pH in the range of 6.5 to 8.5, and which has a molecular weight, determined by gel filtration, between those of cytochrome C and ovalbumin.

REFERENCES:
patent: 3267006 (1966-08-01), Hakim et al.
Chemical Abstracts, vol. 87, 1977: 197039v.
Chemical Abstracts, vol. 92, 1980: 54005y.
Biochim. Biophys. Acta, vol. 122 (1966), pp. 557-559.
Biochemistry, vol. 3, No. 11, 1964, pp. 1737-1741.

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