Thermococcus barossii DNA polymerase mutants

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435194, C12N 912, C12P 1934

Patent

active

058829040

ABSTRACT:
A Thermococcus barosii DNA polymerase with reduced 3'-5' exonuclease activity is disclosed. Additionally disclosed is a Thermococcus barosii DNA polymerase with increased ability to incorporate ribonucleotides and dideoxynucleotides.

REFERENCES:
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patent: 5489523 (1996-02-01), Mathur
patent: 5602011 (1997-02-01), Luhm et al.
Z. Guo, et al., "Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports," Nucl. Acids Res. 22(24):5456-5465, 1994.
T.T. Nikoforov, et al., "Genetic Bit Analysis: a solid phase method for typing single nucleotide polymorphisms," Nucl. Acids. Res. 22(20):4167-4175, 1994.

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