Thermally stable and pH stable subtilisin analogs and method for

Details Thermally stable and pH stable subtilisin analogs and method for Thermally stable and pH stable subtilisin analogs and method for

1991-01-09

1995-03-21

Wax, Robert A.

Compositions

Water-softening or purifying or scale-inhibiting agents

435221, 435222, 4351721, 935 14, C12N 954, C12N 956, C11D 1700

Patent

active

053992830

ABSTRACT:
A mutated subtilisin suitable for admixture to washing compositions and exhibiting substantially improved stability over naturally occurring Bacillus serine proteases is prepared by expressing a modified gene encoding subtilisin in Bacillus subtilis. A preferred subtilisin analog product differs from wild-type Bacillus alkaline proteases by having any amino acid, and preferably serine, at position 218 in place of asparagine. The product is preferably produced in a strain of B. subtilis which is mutated to block synthesis of endogenous proteases. The method of replacing an Asn or a Gly in an Asn-Gly sequence in order to improve pH and thermal stability may be applied to other sites in subtilisin and to other proteins as well.

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