Thermal destabilization of bacillus serine proteases

Details Thermal destabilization of bacillus serine proteases Thermal destabilization of bacillus serine proteases

1981-03-09

1983-05-31

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435188, 435219, 435222, C12N 954, C12N 996, C12N 950, C12N 956

Patent

active

043861601

ABSTRACT:
Bacillus serine proteases are acylated with an acyl radical of a monocarboxylic or dicarboxylic acid of about 1 to 6 carbon atoms to thermally destabilize the proteases at least about 3.degree. C. The acylated proteases have at least about 50% of their activity before acylation, and are advantageous in processes where it is desired to inactivate the proteases at a certain point in the process.

REFERENCES:
patent: 3723250 (1973-03-01), Aunstrup et al.
patent: 3886042 (1975-05-01), Blumberg et al.
patent: 4255454 (1981-03-01), Branner-Jorgensen
Vratsanos S. M., On the Mechanism of Enzyme Action, LXXI, Acylations of Trypsin in Organic Solvents, Archives of Biochemistry and Biophysics, vol. 90, 1960 (pp. 132-138).

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