Thermal cycler including a temperature gradient block

Details Thermal cycler including a temperature gradient block Thermal cycler including a temperature gradient block

2005-11-08

2005-11-08

Fredman, Jeffrey (Department: 1637)

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

C435S004000, C435S288400, C422S063000, C422S067000, C422S091000

Reexamination Certificate

active

06962821

ABSTRACT:
A method in which a temperature gradient is generated across a “gradient” block, and an apparatus comprising a block across which a temperature gradient can be generated. By setting up such a gradient, multiple reaction mixtures held in wells on the gradient block can be simultaneously run at temperatures which differ only slightly, thereby permitting an optimum temperature for the reaction to be quickly identified. In a preferred embodiment the gradient block is integrated into a thermal cycler used for nucleic acid amplification reactions.

REFERENCES:
patent: 3801467 (1974-04-01), Nobe et al.
patent: 4679615 (1987-07-01), Livne
patent: 4865986 (1989-09-01), Coy et al.
patent: 4981801 (1991-01-01), Suzuki et al.
patent: 5038852 (1991-08-01), Johnson et al.
patent: 5207987 (1993-05-01), Kureshy et al.
patent: 5240857 (1993-08-01), Lahetkangas
patent: 5255976 (1993-10-01), Connelly
patent: 5366896 (1994-11-01), Margrey et al.
patent: 5430957 (1995-07-01), Eigen et al.
patent: 5443791 (1995-08-01), Cathcart et al.
patent: 5525300 (1996-06-01), Danssaert et al.
patent: 5779981 (1998-07-01), Danssaert et al.
patent: 6054263 (2000-04-01), Danssaert et al.
patent: 0 209 722 (1998-11-01), None
patent: WO 92 04979 (1992-04-01), None
Advertisement for Stratagene ROBOCYCLER™ Temperature Cycler, Jun. 1993, 1 page.
Advertisement—MJ Research. Inc. Peltier-effect Thermal Cyclers, Jul. 1993, 1 page.
Advertisement—Appligene Crocodile™ II Reactor, Jul. 1992, 1 page.
Advertisement—Ericomp PowerBlock™ Easy Cycler™, 2 pages.
Advertisement—Biometra UNO-Thermoblock and TRIO-Thermoblock, 1993, 1 page.
Advertisement—Corbett Research Fast Capillary Thermal Sequences, 1993, 1 page.
Advertisement—BioTherm Corporation—BioOven II. 1 page. 1992.
Perkin Elmer Cetus GeneAmp PCR System 9600 Brochure, Jul., 1992, 10 pages.
Perkin Elmer Cetus DNA Amplification System Brochures. Apr. 1990. 11 pages.
Advertisement—Inotech Quarter Bath Immersion Thermal Cycler—1 page.
Advertisement—Twin Block™ Thermalcycler, Jan., 1993, 1 page.
Battley, E. H., “A thermal-gradient block for the determination of temperature relationships in microorganisms”,Antonic van Leeuwenhock30:81-96, 1964.
Elliott, R.P., “Temperature-gradient incubator for determining the temperature range of growth of microorgansms”,J. Bacteriology85:889-894, 1963.
Matches, J.R., and Liston. J., “Temperature-gradient incubator for the growth of clostridia”,Can. J. Microbiol., 19:1161-1165, 1973.
Nakae, T.. “Method of temperature-gradient incubation and its application to microbiological examinations”,J Bacteriology91:1730-1735, 1966.
Okami, Y. and Sasaki, Y., “Temperature gradient tools”,Applied Microbiology. 15:1252-1255, 1967.
Oppenheimer. C.H. and Drost-Hansen, W., “A relationship between multiple temperature optima for biological systems and the properties of water”,J. Bacteriology80:21-24, 1960.
Williams, J. Fenton, “Optimization Strategies for the Polymerase Chain Reaction”,BioTechniques, 7(7):762-768. 1989.
Wittwer, Carl T. and Garling, David J., “Rapid Cycle DNA Amplification: Time and Temperature Optimization”,BioTechiques, 10(1):76-83, 1991.

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