Thermal cycler including a temperature gradient block

Details Thermal cycler including a temperature gradient block Thermal cycler including a temperature gradient block

1996-04-19

1998-07-14

Walsh, Stephen

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Means for analyzing gas sample

422 67, 422 63, 422104, 435809, 4352851, 4352861, 4352862, 4352884, 4353031, 935 85, B01L 700

Patent

active

057799814

ABSTRACT:
A method in which a temperature gradient is generated across a "gradient" block, and an apparatus comprising a block across which a temperature gradient can be generated. By setting up such a gradient, multiple reaction mixtures held in wells on the gradient block can be simultaneously run at temperatures which differ only slightly, thereby permitting an optimum temperature for the reaction to be quickly identified. In a preferred embodiment the gradient block is integrated into a thermal cycler used for nucleic acid amplification reactions.

REFERENCES:
patent: 3801467 (1974-04-01), Nobe et al.
patent: 4981801 (1991-01-01), Suzuki et al.
patent: 5038852 (1991-08-01), Johnson et al.
patent: 5255976 (1993-10-01), Connelly
patent: 5525300 (1996-06-01), Danssaert et al.
Advertisement for Stratagene Robocycler.TM. Temperature Cycler, 1 page, Jun. 1993.
Advertisement--MJ Research, Inc. Peltier-effect Thermal Cyclers--1 page, Jul. 1993.
Advertisement--Appligene Crocodile .TM. II Reactor, 1 page, Jul. 1992.
Advertisement--Ericomp PowerBlock.TM. EasyCycler.TM., 2 pages, undated.
Advertisement--Biometra UNO-Thermoblock and TRIO-Thermoblock, 1 page, 1993.
Advertisement--Corbitt Research Fast Capillary Thermal Sequencer, 1 page, 1993.
Advertisement--BioTherm Corporation--BioOven II, 1 page, 1992.
Perkin Elmer Cetus GeneAmp PCR System 9600 Brochure, 10 pages, Jul. 1991.
Perkin Elmer Cetus DNA Amplification System Brochures, 11 pages, Apr. 1990.
Advertisement--Inotech Quarter Bath Immersion Thermal Cycler--1 page, undated.
Advertisement--TwinBlock.TM. Thermalcycler, 1 page, Jan. 1993.

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