Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1997-12-12
2000-07-04
Fitzgerald, David L.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
514 2, 514 12, 530399, 43525421, A61K 3818
Patent
active
060839104
ABSTRACT:
Methods for improving purification and quantification of platelet derived growth factor (PDGF) proteins having structural heterogeneity are provided. Preparation of substantially pure isoforms of these proteins is achieved using TSK sulfopropyl cation exchange chromatography and reverse phase high performance liquid chromatography. A reverse charged capillary zone electrophoresis method enables quantification of substantially pure isoforms of these proteins resulting from endoproteolytic post-translational modifications. Compositions of the invention are substantially purified isoforms of secreted PDGF proteins having structural heterogeneity, more particularly purified intact, single-clipped, and double-clipped isoforms of recombinant PDGF-BB. Pharmaceutical compositions comprising at least one of these substantially purified recombinant PDGF isoforms and methods for their use in promoting wound healing are also provided.
REFERENCES:
Cook et al., "Purification and Analysis of Proteinase-Resistant Mutants of Recombinant Platelet-Derived Growth Factor-BB Exhibiting Improved Biological Activity," The Biochemical Journal, vol. 281, 1 (Jan. 1992), pp. 56-65.
Oefner et al., "Crystal Structure of Human Platelet-Derived Growth Factor BB," EMBO Journal, vol. 11 (1992) pp. 3921-3926.
Kunitani Michael
Parker Hugh
Tran An D.
Alexander Lisa E.
Blackburn Robert P.
Chiron Corporation
Fitzgerald David L.
Henry Leslie T.
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