Drug – bio-affecting and body treating compositions – Lymphokine – Interleukin
Patent
1995-06-06
1996-08-06
Draper, Garnette D.
Drug, bio-affecting and body treating compositions
Lymphokine
Interleukin
435 6952, 530351, A61K 3820
Patent
active
055431410
ABSTRACT:
The present invention relates to recombinant human interleukin-3 (hIL-3) variant or mutant proteins (muteins) in which segments of the polypeptide sequence of the human IL-3 polypeptide have been replaced by segments of the murine (mouse) interleukin-3 (mIL-3) polypeptide to form human/murine chimeric hybrid polypeptides. The human/mouse IL-3 may have amino acid deletions at the N-terminus or the C-terminus or at both the N- and C- termini and in some cases may also contain additional amino acid substitutions or deletions. The human/murine IL-3 muteins retain at least one biological activity of native hIL-3 and may also exhibit an improved side effects profile such as a reduction in the stimulation of leukotriene release or histamine release. The invention also relates to pharmaceutical compositions containing the h/m IL-3 hybrids and methods for using them. Additionally, the present invention relates to recombinant expression vectors comprising nucleotide sequences encoding the IL-3 hybrid muteins, related microbial expression systems, and processes for making the IL-3 hybrids using the microbial expression systems.
REFERENCES:
patent: 4877729 (1989-10-01), Clark et al.
patent: 4959455 (1990-09-01), Clark et al.
patent: 5032395 (1991-07-01), Clark et al.
Ian Clark-Lewis et al, Proc. Nat'l Acad. Sci., 85, 7897-7901 (1988).
Ian Clark-Lewis et al, Science, 231, 134-139 (1986).
T. Yokota et al, Proc. nat'l Acad. Sci., 81, 1070-1074 (1984).
M. C. Fung, Nature, 307, 233-237 (1984).
L. Dorssers et al, Gene, 55, 115-124 (1987) Netherlands.
J. A. Phillips et al, Gene, 84, 501-507, (1989) Netherlands.
Ian Clark-Lewis et al. "Struct.-Function Studies of Interleukin-3 Using an Automated Peptide Syn. Approach", 323-334, Alan R. Liss Inc. (1987) USA.
N. A. Lokker et al, The Journal of Biological Chemistry, 266 1062-1063, -(1991).
James N. Ihle et al, The Journal of Immunology, 126, No. 6, 2184-2189 (1981).
Lambert Dorssers et al, The Journal of Biological Chemistry 266, No. 31, 21310-21317 (1991).
Yu-Chung Yang et al, "Human IL-3: Identif. by Expression Cloning of a Novel Hematopoietic Growth Factor Related to Murine IL-3", Cell, 47, 3-10 (1986).
K. Kaushansky et al, J. Clin., Invest., 90, 1879-1888 (1992).
A. F. Lopez et al, Proc. Nat'l Acad. Sci., 89, 11842-11846 (1992).
Braford-Goldberg Sarah R.
Easton Alan M.
Klein Barbara K.
McKearn John P.
Olins Peter O.
Bennett Dennis A.
Draper Garnette D.
Fitzgerald David L.
G.D. Searle & Co.
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