Multicellular living organisms and unmodified parts thereof and – Nonhuman animal – Transgenic nonhuman animal
Reexamination Certificate
2006-06-06
2006-06-06
Guzo, David (Department: 1636)
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
Transgenic nonhuman animal
C800S003000, C536S024100, C435S320100
Reexamination Certificate
active
07057086
ABSTRACT:
A method of testing a candidate composition for PAI-1 inhibition activity is disclosed. The method includes the steps of obtaining a transgenic non-human warm blooded vertebrate animal having incorporated into its genome a PAI-1 gene encoding a biologically active PAI-1 polypeptide, the PAI-1 gene being present in the genome in a copy number effective to confer over-expression in the transgenic non-human animal of the PAI-1 polypeptide; administering the composition to the transgenic non-human animal; and observing the transgenic non-human animal for determination of a change in the transgenic non-human animal indicative of inhibition of the activity of PAI-1. A transgenic non-human animal useful in such a method is also disclosed, as is a PAI-1 transgene construct encoding a biologically active PAI-1 polypeptide useful for preparing the transgenic non-human animal.
REFERENCES:
patent: 5422245 (1995-06-01), Nielsen et al.
patent: 5980938 (1999-11-01), Berg et al.
patent: WO 90/13648 (1990-11-01), None
Hammer et al. Genetic engineering of mammalian embryos. J. Anim. Sci. 63:269-278, 1986.
Ebert et al. A Moloney MLV-rat somatotropin fusion gne produces biologically active somatotropin in a transgenic pig. Molecular Endocrinology 2:277-283, 1988.
Strojek et al. The use of transgenic animal techniques for livestock improvement. In: Genetic Engineering: Principles and methods, vol. 10, pp. 221-246, 1988.
Kappel et al. Regulating gene expression in trangenic animals. Current Opinion in Biotechnology 3:548-553, 1992.
Mullins et al. Perspective Series: Molecular medicine in genetically engineered animals. J. Clin. Invest. 98: S37-S40, 1996.
Wall, R.J. Transgenic livestock: Progress and prospects for the future. Theriogenology 45:57-68, 1996.
Lijnen et al. Nutritionally induced obesity is attenuated in transgenic mice overexpressing plasminogen activator inibitor-1.
Harats et al. Targeting gene expression to the vascular wall in transgenic mice using the murine preproendothelin-1 promoter. J. Clin. Invest. 95:1335-1344, 1995.
Erickson et al., Development of venous occlusions in mice transgenic for the plasminogen activator inhibitor-1 gene,Nature346:74-76 (Jul. 5, 1990).
Lyons-Giordano et al., Skin Abnormalities in Mice Transgenic for Plasminogen Activator Inhibitor 1: Implications for the Regulation of Desquamation and Follicular Neogenesis by Plasminogen Activator Enzymes,Development of Biology170:289-298 (1995).
Eitzman et al., Bleomycin-induced Pulmonary Fibrosis in Transgenic Mice That either Lack or Overexpress the Murine Plasminogen Activator Inhibitor-1 Gene,J. Clin. Invest. 97(1):232-237 (Jan. 1996).
Yamazaki et al., “Hair Cycle-Dependent Expression of Hepatocyte Growth Factor (HGF) Activator, Other Proteinases, and Proteinase Inhibitors correlates with the Expression of HGF in Rat Hair Follicles”Journal for Investigative Dermatology Symposium Proceedings,4: 312-315, 1999.
Vleugels et al., “Evaluation of the Mechanism of Inactivation of Plasminogen Activator Inhibitor-1 by Monoclonal Antibodies Using a Stable Variant”,Fibrinolysis & Proteolysis. 12 (5): 277-282, 1998.
Pannekoek et al., “Endothelial Plasminogen Activator Inhibitor (PAI): A New Member of the Serpin Gene Family”The EMPO Journal,5(10): 2539-2544, 1986.
Eren et al., “Structure/Function Relationships and Phenotype in PAI-1 Transgenic Mice”,Circulation,106(19) Supplement: II-40, Nov. 5, 2002.
Declerck Paul J.
Eren Mesut
Vaughan Douglas E.
Guzo David
Jenkins & Wilson & Taylor, P.A.
Nguyen Quang
Vanderbilt University
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