Theophylline antigens and antibodies

Details Theophylline antigens and antibodies Theophylline antigens and antibodies

1980-04-07

1981-04-14

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435810, 435188, 424 12, 23230B, C12Q 166, C12N 996

Patent

active

042620899

ABSTRACT:
Theophylline derivatives are provided for preparing reagents for use in competitive protein binding assays and for use as reagents in competitive protein binding assays. Theophylline is substituted at the 3 position and conjugated to antigens for production of antibodies which specifically recognize theophylline as distinct from structurally similar analogs such as caffeine. Enzyme conjugates are provided which find use for measuring the amount of theophylline in a sample suspected of containing theophylline. A method is provided employing the reagents for the determination of theophylline.

REFERENCES:
patent: 4067774 (1978-01-01), Rubenstein
Cook et al., "Theophylline Radio Immunoassay Synthesizing Antigen and Characterization of Antiserum", Chem. Abstracts, vol. 84, No. 21, (1976), pp. 8, 9, Abs. No. 144484t.
Nishikawa et al., "Competitive Nephelometric Immunoassay of Theophylline in Plasma, Clin. Chim Acta, vol. 91, No. 1, (1979), pp. 59-65.
Long, "Enzyme Immunoassay with Use of Centrifugal Analyzer of Phenytoin, Phenobarbital and Theophylline", _Clin Chem., vol. 24, No. 2, (1978), p. 391.
Schuurs et al., "Enzyme-Immunoassay", Clin Chim Acta, vol. 81, (1977), pp. 1-40.

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