Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1989-09-11
1992-05-26
Nucker, Christine
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 4, 435 25, 435 29, 435960, 548250, 548252, 548254, C12Q 132, C12Q 100, C02D25704, C02D25710
Patent
active
051167328
ABSTRACT:
Compounds of the formula DNB-TZ(P2) (P3) X, wherein TZ is a tetrazolium ring, DNB is 5-(2,4-dinitrophenyl), X is halide and P2 and P3 are each independently halophenyl, nitrophenyl or phenyl. The compound INDT 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-dinitrophenyl) tetrazolium bromide exhibits facile reduction to insoluble chromophoric formazan compared to the chromagen INT. INDT differs structurally from INT 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride by having 2,4-dinitrophenyl instead of phenyl at the 5-position on the tetrazolium ring. The compounds, including INDT, are useful as chromagens for histological staining, as well as in enzyme-amplified staining as a part of immunological or hybridization assays.
REFERENCES:
patent: 3881930 (1975-05-01), Schleigh
patent: 3887372 (1975-06-01), Bailey
patent: 3887374 (1975-06-01), Brongo et al.
patent: 3957514 (1976-05-01), Adin
patent: 4284704 (1981-08-01), Fleming et al.
patent: 4731335 (1988-03-01), Brigati
patent: 4792521 (1988-12-01), Shochat
Chemical Abstract CA 110(23):208931b 1987.
E. Seidler, "New nitro-monotetrazolium salts and their use in histochemistry" Histo chem. Journal, vol. 12, pp. 619-630 (1980).
F. P. Altman,"Tetrazolium Salts and For Formazans", Prog. Histochem. Cytochem. vol. 9, pp. 1-56 (1976).
F. P. Altman, "Tetrazolium salts: a consumer's guide," Histochem. Journal, vol. 8, pp. 471-485 (1976).
E. P. Unger et al., "Automation of in situ hybridization" J. of Histotechnology, vol. 11, No. 4, pp. 253-258 (1988).
Arvanaghi Massoud
Brigati David J.
Nagubandi Sreeramulu
Doernberg Alan M.
Fisher Scientific Co.
Nucker Christine
Preston David R.
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