Tetracycline resistance protein

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 693, 435 701, 435 711, 435 712, 4353201, 435883, 536 237, 536 2432, C12N 1511, C12N 1563, C12N 120

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active

058916709

ABSTRACT:
The invention provides tetracycline resistance protein polypeptides and DNA (RNA) encoding tetracycline resistance protein polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing tetracycline resistance protein polypeptides to screen for antibacterial compounds.

REFERENCES:
van der Lelie, D., Bron, S. Venema, G. and Oskam, L. GenBank Submission, Accession No. P13924.
Eisel et al. Tetanus toxin: primary structure, expression in E. coli, and homology with botulinum toxins, EMBO J. 5:2495-2502, 1986.
Lacks et al. Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1, J. Mol. Biol. 192:753-765, 1986.
Herzog et al. Molecular cloning, characterization, and localization of the human homolog to the reported bovine NPY Y3 receptor: lack of NPY binding and activation, DNA and Cell Biol. 12:465-471, 1993.
Wojdani et al. Isolation and characterization of tetracycline resistance proteins from Staphylococcus aureus and Escherichia coli, Antimicrob. Agents Chemother. 9:526-534, 1976.
Guay et al. The tet(K) gene from Staphylococcus aureus mediates the transport of potassium in Escherichia coli, J. Bacteriol. 175:4927-4929, 1993.
Fujihira et al. Transmembrane glutamic acid residues play essential roles in the metal-tetracycline/H+antiporter of Staphylococcus aureus, FEBS Lett. 391:243-246, 1996.
Sambrook et al. Molecular Cloning, Expression of Cloned Genes in E. coli, pp. 17.1-17.44, 1989.
van der Lelie, D., Bron, S. Venema, G. and Oskam, L. GenBank Submission, Accession No. P13924, created 01 Jan. 1990.

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