Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2005-07-05
2005-07-05
Shukla, Ram R. (Department: 1632)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Reexamination Certificate
active
06914124
ABSTRACT:
A method for regulating expression of a tet operator-linked gene in a cell of a subject is disclosed. In one embodiment, the method involves introducing into the cell a nucleic acid molecule encoding a tetracycline-controllable transactivator (tTA), the tTA comprising a Tet repressor operably linked to a polypeptide which directly or indirectly activates transcription in eucaryotic cells; and modulating the concentration of a tetracycline, or analogue thereof, in the subject. Alternatively, in another embodiment, the method involves obtaining the cell from the subject, introducing into the cell a first nucleic acid molecule which operatively links a gene to at least one tet operator sequence, introducing into the cell a second nucleic acid molecule encoding a tTA, to form a modified cell, administering the modified cell to the subject, and modulating the concentration of a tetracycline, or analogue thereof, in the subject. The first and second nucleic acid molecule can be within a single molecule (e.g., in the same vector) or on separate molecules.
REFERENCES:
patent: 4833080 (1989-05-01), Brent et al.
patent: 5221778 (1993-06-01), Byrne et al.
patent: 5464758 (1995-11-01), Gossen et al.
patent: 5545808 (1996-08-01), Hew et al.
patent: 5595895 (1997-01-01), Miki et al.
patent: 6136954 (2000-10-01), Bujard et al.
patent: 0 332 416 (1989-09-01), None
patent: 0 455 424 (1991-11-01), None
patent: 0 455 687 (1991-11-01), None
patent: 0 494 724 (1992-07-01), None
patent: WO 91/13979 (1991-09-01), None
patent: WO 91/19784 (1991-12-01), None
patent: WO 91/19796 (1991-12-01), None
patent: WO 92/11874 (1992-07-01), None
patent: WO 92/20808 (1992-11-01), None
patent: WO 93/04169 (1993-03-01), None
patent: WO 93/23431 (1993-11-01), None
patent: WO 94/04672 (1994-03-01), None
patent: WO 94/18317 (1994-08-01), None
patent: WO 94/29442 (1994-12-01), None
patent: WO 96/01313 (1996-01-01), None
Ackland-Berglund, C.E. and Leib, D.A. (1995)BioTechniques 19:216-217.
Ackland-Berglund, C.E. and Leib, D.A. (1995) “Efficacy of Tetracycline-Controlled Gene Expression Is Influenced by Cell Type”BioTechniques 18(2):196-200.
Agarwal, M.L. et al. (1995) “p53 Controls Both the G2/M and the G1Cell Cycle Checkpoints and Mediates Reversible Growth Arrest in Human fibroblasts,”Proceedings of the National Academy of Science, Sci USA, 92:8493-8497.
Altschmied, L. et al. (1988) “A threonine to alanine exchange at position 40 of Tet repressor alters the recognition of the sixth base pair of tet operator from GC to AT”,The EMBO Journal, 7(12):4011-4017.
Baim, S.B. et al. (1991) “A chimeric mammalian transactivator based on the lac repressor that is regulated by temperature and isopropyl β-D-thiogalactopyranoside”,Proceedings of the National Academy of Science 88:5072-5076.
Baniahmad, A. et al. (1991) “A Transferable Silencing Domain Is Present In the Thyroid Hormone Receptor, In the v-erbA Oncogene Product and In the Retinoic Acid Receptor”The EMBO Journal 11(3):1015-1023.
Baumeister, R. et al. (1992) “Tet Repressor Tet Interactions Derived From Mutants With New Recognition Specificities”,Structural Tools For The Analysis Of Proten-Nucleic Acid Complexes Advances in Life Sciences,Birkhauser, Basel ;Boston, pp. 175-183.
Baumeister, R. et al., (1992) “Contacts Between Tet Repressor And Tet Operator Revealed By New Recognition Specificities Of Single Amino Acids Replacement Mutants”,Journal Of Molecular Biology, 226:1257-1270.
Baumeister, R. et al. (1992) Functional Roles of Amino Acid Residues Involved In Forming THE.Alpha.-helix-turn-.alpha.-helix operator DNA Binding Motif Of Tet repressor From Th10,Proteins: Structure, Function, and Genetics, 14(2):168-177.
Bergman, M. et al. (1995) “Overexpressed Csk Tyrosine Kinase Is Localized in Focal Adhesions, Causes Reorganization of αvβ5Integrin, and Interferes with HeLa Cell Spreading”,Molecular and Cellular Biology, 15(2):711-722.
Boshart, M. et al. (1985) “A Very Strong Enhancer Is Located Upstream of an Immediate Early Gene of Human Cytomegalovirus”,Cell 41(2):521-530.
Bradley, A. (1991) “Modifying the mammalian genome by genet targeting”,Current Opinion in Biotechnology 2:832-829.
Bradley, A. (1992) “Modifying The Mouse: Design And Desire”,Biotechnology, 10:534-539.
Brent, R. and M. Ptashne (1984) “A Bacterial Repressor Protein or a Yeast Transcriptonal Terminator Can Block Upstream Activation of A Yeast Gene”Nature 312:612-615.
Brent R. and M. Ptashne (1985) “A Eukaryotic Transcriptional Activator Bearing the DNA Specificity of a Prokaryotic Repressor”Cell 43:729-736.
Brown, M., et al. (1987) “lac Repressor Can Regulate Expression from a Hybrid SV40 Early Promoter Containing a lac Operator in Animal Cells”,Cell 49:603-612.
Buckbinder L. et al. (1994) “Gene Regulation by Temperature-Sensitive p53 Mutants: Identification of p53 response genes”Proc. Natl. Acad. Sci. USA 91:10640-10644.
Capecchi, M.R. (1989) “Altering the Genome by Homologous Recombination”,Science 244:1288-1292.
Cayrol, C. et al. (1995) “Identification of Cellular Target Genes of the Epstein-Barr Virus Transactivator Zta: Activation of Transforming Growth Factor βigh3 (TGF-βigh3) and TGF-β1”,Journal of Virology, 69(7):4206-4212.
Chen, Y.Q. et al. (1995) “Tumor Suppression by p21WAF11”,Cancer Research, 55:4536-4539.
Coghlan, A. (1995) “Gene drream fades away”New Scientist 148:14-15.
Courey, A.J. and Tjian, R., (1988) “Analysis of Sp1 In Vivo Reveals Multiple Transcriptional Domains, Including a Novel Glutamine-Rich Activation Motif”,Cell 55:887-898.
Cowell, (1994) “Repression versus activation in the control of gene transcription,”Trends in Biochemical Sciences,19:1, 38-42.
Crystal, R.G. (1995) “Transfer of Genes to Humans: Early Lessons and Obstacles to Success”,Science 270:404-410.
Daddona et al. (1984) “Human Adenosine Deaminase.”J. Biol. Chem. 259:12101-12106.
Damke, H. et al. (1994) “Induction of Mutant Dynamin Specifically Blocks Endocytic Coated Vesicle Formation,”The Journal of Cell Biology 127(4):915-934.
Damke, H. et al. (1995) “Tightly Regulated and Inducible Expression of Dominany Intefering Dynamin Mutant in Stably Transformed HeLa Cells.”Methods in Enzymology 257:209-220.
Degenkolb, J. et al. (1991) “Sructural Requirements of Tetracycline-Tet Repressor Interaction: Determination of Equilibrium Binding Constants for Tetracycline Analogs with the Tet Repressor”Antimicrobial Agents and Chemotherapy 35(8):1591-1595.
Deuschle, u., et al. (1989) “Regulated expression of foreign genes in mammalian cells under the control of coliphage T3 RNA polymerase and lac repressor”,Proceedings of the National Academy of Science USA 86:5400-5404.
Deuschle et al. (1995) “Tetracycline-reversible silencing of eukaryotic promoters,”Mol. Cell. Biol., 15:4, 1907-1914.
Dhawan, J. et al. (1995) “Tetracyclin-Regulated Gene Expression Follwing Direct Gene Transfer into Mouse Skeletal Muscle”,Somatic Cell and Molecular Genetics, 21(4):233-240.
Ebert, K.M. et al. (1988) “A Moloney MLV-Rat Somatotropin Fusion Gene Produces Biologically Active Somatotropin in a Transgenic Pig.”Molecular Endocrinology 2(3):277-283.
Efrat, S. et al., (1995) “Conditional Transformation of a Pancreatic β-Cell Line Derived From Transgenic Mice Expressing A Tetracyclin-Regulated Oncogene”PNAS. USA, 92:3576-3580.
Englert, C. et al. (1995) “WT1 suppresses synthesis of the epidermal growth factor receptor and induces apoptosis,”EMBO journal, 14(19):4662-4675.
Epstein-Baak, R. et al. (1992) “Inducible Transformation of Cells from Transgenic Mice Expressing SV40 under Lac Operon Control”,Cell Growth&Differentiation 3:127-134.
Fieck, A. et al. (1992) &
Bujard Hermann
Gossen Manfred
Lahive & Cockfield LLP
Shukla Ram R.
Smith DeAnn F.
TET Systems Holding GmbH & Co. KG
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