Tethered pair fluorescence energy transfer indicators, chemical

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample

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128634, 2504831, 422 57, 422 58, 422 8207, 422 8211, 436172, G01N 2164, G01N 2177

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active

050376152

ABSTRACT:
A fluorescence energy transfer indicator, a chemical sensor incorporating it, and a method for making it are provided, such being useful in medical, biological and industrial applications. The fluorescence energy transfer indicator includes a rigid spacer group that tethers together a fluorescent energy donor and a colorimetric indicator acceptor, the tethering being across a predetermined, known or determinable length in order to thereby tailor the indicator to the specific needs of the chemical sensor. The emission spectrum of the fluorescent energy donor overlaps with an absorbance spectrum of the colorimetric indicator acceptor, which undergoes a change in color or in color intensity as a function of the species being monitored or measured in accordance with parameters of the species such as pH, oxygen concentration, carbon dioxide concentration and the like.

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