Tethered function assay for protein function

Details Tethered function assay for protein function Tethered function assay for protein function

1998-05-20

1999-11-16

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 241, 53038821, C12Q 168, C07K 1600, C07H 2104

Patent

active

059855754

ABSTRACT:
A method for determining the function of a test protein is disclosed. In a preferred embodiment, this method comprises the steps of fusing a test protein to an RNA-binding protein and exposing the fusion protein to recombinant reporter mRNA molecule that comprises a binding site for the RNA-binding protein in the 3' untranslated region. One then observes the properties of reporter mRNA and correlates the reporter properties with test protein function.

REFERENCES:
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C. Southgate, et al., "Activation of Transcription by HIV-1 Tat Protein Tethered to Nascent RNA Through Another Protein," Nature 345(6276) :640-642, 1990.
R. Stripecke. et al., "Proteins Binding to 5' Untranslated Region Sites: A General Mechanism for Translational Regulation of mRNAs in Human and Yeast Cells," Mole. Cell. Biol. 14(9) :5898-5909, 1994.
J. Valcarcel, et al., "The Protein Sex-Lethal Antagonizes the Splicing Factor U2AF to Regulate Alternative Splicing of Transformer Pre-mRNA," Nature 362:171-175, 1993.
M. Wickens, et al., "Translational Control of Developmental Decisions," Translational Control, Cold Spring Harbor Laboratory Press, pp. 411-450, 1996.
M. Wickens, et al., "Life and Death in the Cytoplasm: Messages from the 3' End," Curr. Opin. Genet. & Develop. 7:220-232, 1997.
Paraskeva et al., Proc. Natl. Acad. Sci. USA, 95(3), 951-956, Feb. 1998.

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