Testis-specific gene

Details Testis-specific gene Testis-specific gene

2001-09-26

2003-04-29

Ketter, James (Department: 1636)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C536S023200, C514S04400A

Reexamination Certificate

active

06555670

ABSTRACT:

BACKGROUND OF THE INVENTION
1. Field of the Invention
This invention relates to a testis-specific gene, and particularly relates to a gene which is located on the X-chromosome and is specifically expressed in haploid cells of the testis, and relates to uses thereof.
2. Description of Related Art
Various genes, such as protamine, have already been reported as genes which are specifically expressed in haploid cells of the testis. However, among these genes, only fsc
1
is known as a gene which is located on the X-chromosome and is specifically expressed in haploid cells of the testis.
In order to carry out chromosomal manipulations of the livestock, gene diagnostics or gene therapy for infertility due to sperm dysplasia, or the like, by means of artificially manipulating gene expression in the testis, or introducing a gene or a recombinant, which can be specifically expressed in haploid cells, it is indispensable that a gene which is located on a sex chromosome and is specifically expressed in the haploid cells of the testis be isolated and be utilized.
SUMMARY OF THE INVENTION
This invention provides a gene specifically expressed in haploid cells of the testis.
This invention provides a gene, which is located on the X-chromosome of a mammal, is specifically expressed in haploid cells of the testis, and encodes an amino acid sequence having a homology of at least 25% with an amino acid sequence encoded by drosophila gcl gene.
The mammal can be selected from the group including mouse, human, and cattle.
This invention also provides a DNA which can hybridize, under stringent conditions, with a DNA including the base sequence in SEQ ID NO: 1 or a DNA sequence containing substitution, deletion, and/or addition of one or more nucleotides in a base sequence in SEQ ID NO: 1.
The invention further provides a protein including an amino acid sequence in SEQ ID NO: 2, or a protein including an amino acid sequence containing substitution, deletion, and/or addition of one or more amino acid residues in the amino acid sequence in SEQ ID NO: 2. The invention further provides an RNA transcribed from the aforementioned DNA.
The invention further provides a method of gene diagnosis by using a base sequence in SEQ ID NO: 1. The invention further provides a method of discrimination of sex by using a base sequence in SEQ ID NO: 1. The invention further provides a method of separation of sperm by using a base sequence in SEQ ID NO: 1 or by using an amino acid sequence in SEQ ID NO: 2. The invention further provides a method of infertility treatment by using a base sequence in SEQ ID NO: 1 or by using an amino acid sequence in SEQ ID NO: 2. The invention further provides a method of chromosomal manipulation by using a base sequence in SEQ ID NO: 1.


REFERENCES:
patent: 99/09168 (1999-02-01), None
patent: 99/27091 (1999-06-01), None
Davisson M.T. X-linked genetic homologies between mouse and man. Genomics. 1987 Nov.;1(3):213-227.*
Graves and Watson Mammalian sex chromosomes: evolution of organization and function. Chromosoma. 1991 Nov.;101(2):63-8.*
Accession, AV041341, EST Nov. 23, 1999.*
Accession, AV260338, EST, Nov. 04, 1999.*
Judith L. Leatherman et al., “Indentification of a mouse germ cell-less homologue with conserved activity in Drosphila” Mechanisms of Development, col. 92, No. 2, Apr. 2000, pp. 145-153.
Database EMBL 'Online! Oct. 1, 1993, Database accession No. QO1820XP002192737, Germ Cell-Less Protein. CGL or CG8411. Drosphila melanogaster (Fruit fly), Abstract.
Database EMBL 'Online! Nov. 2, 2000, Database Accession No. AF198534 SP002192738, Homo sapiens germ cell-less protein (GLC) mRNA, partial cds. Nili E et al., “The human germ cell-less (HGCL): a candidate gene for Alstrom syndrom” unpublished Abstract.
Database EMBL 3 Online! Jun. 1, 2001, Database Accession No. Q99N66 SP002192739 Mouse Germ Cell-Less Haploid Specific. MDCLH Aizawa et al., “Molecular cloning of a novel mouse germ cell-less homologue expressing haploid specifically in mouse testis”.
Tohru Kimura et al., Molecular Cloning and Genomic Organization of Mouse Homologue of Drosphila germ cell-less and Its Expression of Germ Lineage Cells.

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