Testing the operation of a recording fluorometer/densitometer

Details Testing the operation of a recording fluorometer/densitometer Testing the operation of a recording fluorometer/densitometer

1977-05-24

1978-06-27

Willis, Davis L.

Radiant energy

Calibration or standardization methods

250461R, G01D 1800, G01N 2138

Patent

active

040977358

ABSTRACT:
A test sample for testing a recording fluorometer/densitometer has multiple tracks with features that generate detector outputs indicating performance parameters. In the normal mode of operation the instrument scans the optical absorbance or fluorescence characteristics of electrophoretic samples in a plurality of tracks on a thin agarose film plate. In order to correctly record the optical characteristics of these electrophoretic samples, the instrument must respond linearly and with the correct range, resolution and alignment. A test sample is scanned in the same manner as a normal sample. The resulting instrument output provides easily analyzed information on fluorometric and densitometric performance parameters including linearity, range, spatial resolution, slit and scan path alignments, scan speed and excitation uniformities, response time, noise, electrical and optical offsets, integration accuracies, automatic gain and zeroing accuracies.

REFERENCES:
patent: 3706877 (1972-12-01), Clifford, Jr. et al.

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