Chemistry: electrical and wave energy – Apparatus – Electrolytic
Reexamination Certificate
2001-05-18
2002-12-10
Tung, T. (Department: 1743)
Chemistry: electrical and wave energy
Apparatus
Electrolytic
C204S403060, C204S403100, C204S403010, C427S002130
Reexamination Certificate
active
06491803
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a test strip and a biosensor having an increased conductivity with addition metal particles in nanometer size.
2. Description of the Prior Art
Various test strips, including electrochemical biosensor test strips, are used in measuring the amount of an analyte in a fluid. The biosensor test strips can provide rapid assays with a good accuracy so that they can be used in research and clinical field to treat a large number of samples.
U.S. Pat. No. 5,120,420 discloses a biochemical detecting electrode strip, including an electrode portion, an insulating layer, a reaction layer, and a sample receiving space which is formed by laying a resin board and a hydrophilic cover on the reaction layer. The biochemical detecting electrode strip has some disadvantages. Three steps are required in forming the reaction layer, i.e. forming the CMC layer to improve hydrophilic property of the carbon electrode surface, forming the GOD layer, and forming the conductive medium. Each step requires a subsequent drying step. The process is complicated. In addition, there is only one sampling inlet in the electrode strip and the sample is introduced into the reaction area through contacting with an electrode strip tip. Normally, the conductivity and its stability is not good because of the interference of the insulating materials in the test strip.
U.S. Pat. No. 5,508,171 relates generally to a determination of the concentration of analytes in fluids and more specifically to an amperometric biosensor for use in such determination. U.S. Pat. No. 5,727,548 relates to enzymatic sensor electrodes and their combination with reference electrodes to detect a compound in a liquid mixture. U.S. Pat. No. 5,997,817 discloses an improved electrochemical biosensor test strip for includes an indentation for tactile feel as to the location of the strips sample application port. However, there is a problem of poor conductivity and conductive stability caused by the interference materials occuring in the prior art.
There is a need to develop an electrode strip or a biosensor with a superior conductivity in the detection of biomolecules.
SUMMARY OF THE INVENTION
One object of the invention is to provide a test strip for detecting biochemical components in a sample, comprising:
an electrically insulating substrate;
a conducting film coated on one side of said insulting substrate to form an isolated and disconnected anode, and an isolated and disconnected cathode wherein said anode disposed on the substrate, the anode being formed with, on both ends of the anode, a working electrode and an anode connector respectively; and said cathode disposed on the substrate, the cathode being formed with, on both ends of the cathode, a reference electrode and a cathode connector respectively;
a reaction layer wherein said layer is form by coating the working electrode and the reference elect with a slurry comprising a fiber, metal particles having a size in nanometer, and a bioactive substance; and
an electrically insulating layer disposed on the substrate and having an opening for receiving the sample on the reaction layer.
Another object of the invention is to provide a method of producing said test strip of the invention, which comprises the following steps:
(a) coating a conducting film on one side of an electric insulating substrate and forming isolated and disconnected an anode and a cathode;
(b) coating an electric insulating film on a part of the conducting film, wherein one end of an uncovered anode of the conducting film is at least a reference electrode and the other end an anode connector, and one end of an uncovered cathode of the conducting film is at least a working electrode and the other end a cathode connector; and
(c) coating a region containing at least the working electrode, with a the slurry comprising a fiber, metal particles having a size in nanometer and a bioactive substance, and the reference electrode to form a reaction film so as to connect the working electrode and the reference electrode individually.
A further object of the invention is to provide a biosensor, which comprises a test strip of the invention and a detection device.
One object of the invention is to provide a slurry for increasing conductivity of a test strip, which comprises a fiber, a metal particle having a size in nanometer and a bioactive substance.
REFERENCES:
patent: 4970145 (1990-11-01), Bennetto et al.
patent: 5120420 (1992-06-01), Nankai et al.
patent: 5160418 (1992-11-01), Mullen
patent: 5508171 (1996-04-01), Walling et al.
patent: 5727548 (1998-03-01), Hill et al.
patent: 5762770 (1998-06-01), Pritchard et al.
patent: 5770028 (1998-06-01), Maley et al.
patent: 5997817 (1999-12-01), Crismore et al.
Chen Wen-Chang
Chuang Jen-Hung
Lin Hong-Ming
Shen Thomas Y. S.
Apex Biotechnology Corporation
Noguerola Alex
Rothwell Figg Ernst & Manbeck
Tung T.
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