Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1979-06-14
1982-01-19
Nucker, Christine M.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230B, 424 12, 252316, G01N 3356, A61K 4300, B01J 1302
Patent
active
043116906
ABSTRACT:
Disclosed is a process for determining the concentration of unbound hormone, e.g., thyroxine, cortisol, or testosterone, in a liquid sample containing hormone and protein capable of binding the hormone. The sample is incubated with antibody specific to the hormone to be detected and a distinguishable analogue of the hormone, both of which are separated from the sample by semipermeable membranes capable of excluding the passage of natural protein and antibody but which allow passage of the hormone and its analogue. Preferably, the antibody and analogue are contained in semipermeable microcapsules. Free hormone in the sample permeates the membranes and competes for sites of attachment to the antibody with the analogue. The antibody and free hormones are then separated. The amount of analogue present either in association with the antibody or in the remainder of the reaction system is indicative of the level of free hormone originally present in the sample.
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Bueler et al., Clin. Chem., vol. 24, No. 6, Jun. 1978, p. 1040.
Buehler Robert J.
Chan Teresa H.
Lim Franklin
Damon Corporation
Nucker Christine M.
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