Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Reexamination Certificate
2005-04-19
2005-04-19
Horlick, Kenneth R. (Department: 1634)
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
C435S006120, C435S091100, C435S287100, C536S023100, C536S024300, C536S024330
Reexamination Certificate
active
06881570
ABSTRACT:
A plurality of cDNAs each having a known different base sequence are labeled with a fluorescent dye (FITC), whereby F-cDNA is prepared. The F-cDNA is disposed at a plurality of predetermined positions on the slide glass of a DNA micro array chip. Cy5-cDNA is prepared by synthesizing cDNA from poly(A)-mRNA in the presence of a fluorescent dye (Cy5). The Cy5-cDNA is placed on the DNA micro array chip and hybridized to the F-cDNA. After hybridization, the DNA micro array chip is read by a quantitative apparatus incorporated with an analyzer in which information about the base composition ratio and base length of cDNA has been registered.
REFERENCES:
patent: 5631734 (1997-05-01), Stern et al.
patent: 5807522 (1998-09-01), Brown et al.
patent: 5849290 (1998-12-01), Brown et al.
patent: 5974164 (1999-10-01), Chee
patent: 5-20712 (1993-03-01), None
patent: 9703251-0 (1999-04-01), None
Arnold et al., Phosphorylation of the human estrogen receptor on tyrosine 537 in vivo and by Src family tyrosine kinases in vitro, Mol. Endocrinol. 9, 24-33, 1995.*
Larin et al., Fluorescence in situ hybridization of multiple probes on a standard glass microscope slide. Nucleic Acids Res. 22, 3689-3692. 1994.*
Cardullo et al., Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer. Proc. Natl .Acad. Sci. USA, 85, 8790-9794, 1988.*
Pastore et al., A quantitative polymerase chain reaction (PCR) assay completely discriminates between Duchenne and Backer muscular dystrophy deletion carriers and normal females. Mol. Cell. Probes 10, 129-137, 1996.*
Liang et al., Recent advances in differential display. Current Opinion in Immunology 7, 274-280, 1995.*
Guo et al., Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. Nucleic Acids Res. 22, 5456-5465, 1994.*
Stephens et al., Transcriptional repression of the GLUT4 and C/EBP gene s in 3T3-L1 adipocytes by tumor necrosis factor-alpha . J. Biol. Chem. 266, 21839-21845, 1991.*
Smith et al., Automated differential display using a fluorescently labeled universal primer. Biotechniques 23, 274-279, Aug. 1997.
Fuji Photo Film Co. , Ltd.
Horlick Kenneth R.
Lu Frank
LandOfFree
Test piece and quantitative method and apparatus for an... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Test piece and quantitative method and apparatus for an..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Test piece and quantitative method and apparatus for an... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3388175