Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1981-10-30
1984-05-01
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 34, 435 37, 435253, 435871, C12Q 168, C12Q 104, C12Q 112, C12N 120
Patent
active
044462301
ABSTRACT:
A strain of Neisseria gonorrhoeae ATCC 31953 is described which is abnormal in that it has characteristically poor growth on chocolate agar at a temperature range of about 30.degree. C. to about 37.degree. C. in a CO.sub.2 atmosphere suitable for growth of N. gonorrhoeae. This strain is resistant to nalidixic acid at the 5-10 mcg/ml level and resistant to streptomycin at the 1000 mcg/ml level or greater. N. gonorrhoeae ATCC 31953 is a test strain suitable for use in the method described for the laboratory diagnosis of gonorrhea. The method comprises the steps of (1) applying a non-toxic preparation of a patient's specimen material, directly to a culture of Neisseria gonorrhoeae ATCC 31953, which has abnormal growth characteristics, which is in or on a biological medium suitable for growth of normal Neisseria gonorrhoeae, and observing for the restoration of normal growth to the abnormal growth strain Neisseria gonorrhorae ATCC 31953, in or on the biological medium of step (1), under conditions normal for growth of Neisseria gonorrhoeae. The observence of growth indicates positive detection of N. gonorrhoeae DNA.
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Temple University Of The Commonwealth System Of Higher Education
Warden Robert J.
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