Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2005-04-01
2008-03-18
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C435S038000
Reexamination Certificate
active
07344854
ABSTRACT:
A test medium and method for detecting, quantifying, identifying and differentiating up to four (4) separate biological materials in a test sample. A test medium is disclosed which allows quantifying and differentiating under ambient light aggregates of biological entities producing specific enzymes, which might include general coliforms,E. coli, Aeromonas, andSalmonellain a single test medium. A new class of nonchromogenic substrates is disclosed which produce a substantially black, non-diffusible precipitate. This precipitate is not subject to interference from other chromogenic substrates present in the test medium. In one embodiment, the substrates are selected such thatE. colicolonies present in the test medium show as substantially black, general coliforms colonies show in the test medium as a blue-violet color,Aeromonascolonies present in the test medium show as a generally red-pink color, andSalmonellacolonies show as a generally teal-green color. Other microorganisms and color possibilities for detection and quantification thereof are also disclosed. An inhibitor and method for making a test medium incorporating the inhibitor are disclosed.
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Roth Geoffrey N
Roth Jonathan N
Baker & Daniels LLP
Gitomer Ralph
Micrology Laboratories, LLC
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