Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1993-03-01
1995-02-28
Knode, Marian
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, 435 34, 4352528, C12Q 110, C12Q 104
Patent
active
053936620
ABSTRACT:
A chromogenic .beta.-galactosidase substrate producing an insoluble precipitate of a first color when reacted upon by .beta.-galactosidase and a chromogenic .beta.-glucuronidase substrate producing an insoluble precipitate of a second, contrasting color when reacted upon by .beta.-glucuronidase are combined in a test medium for quantitatively identifying and differentiating general coliforms and E. coli. The .beta.-galactosidase substrate 5-bromo-4-chloro-3-indolyl-.beta.-D-galactopyranoside which produces an indigo blue precipitate when reacted upon by .beta.-galactosidase may be used with one of the novel compounds 6-chloroindolyl-.beta.-D-glucuronide, 4,6-dichloroindolyl-.beta.-D-glucuronide, 6,7-dichloroindolyl-.beta.-D-glucuronide, and 4,6,7-trichloroindolyl-.beta.-D-glucuronide, which produce mauve or magenta precipitates when reacted upon by .beta.-glucuronidase. The .beta.-glucuronidase substrate 5-bromo-4-chloro-3-indolyl-.beta.-D-glucuronide, which produces an indigo blue precipitate when reacted upon by .beta.-glucuronidase may be used with one of the novel compounds 6-chloroindolyl-.beta.-D-galactoside, 4,6-dichloroindolyl-.beta.-D-galactoside, 6,7-dichloroindolyl-.beta.-D-galactoside, and 4,6,7-trichloroindolyl-.beta.-D-galactoside which produce mauve or magenta precipitates when reacted upon by .beta.-galactosidase.
REFERENCES:
patent: 3496066 (1970-02-01), Berger et al.
patent: 3870601 (1975-03-01), Warren et al.
patent: 3936356 (1976-02-01), Janin
patent: 3957584 (1976-05-01), Kronish et al.
patent: 4351823 (1982-09-01), Rubin
patent: 4503143 (1985-03-01), Gerber et al.
patent: 4556636 (1985-12-01), Belly et al.
patent: 4584368 (1986-04-01), Rubin
patent: 4591554 (1986-05-01), Koumura et al.
patent: 4603108 (1986-07-01), Bascomb
patent: 4668622 (1987-05-01), Kuhr
patent: 4673638 (1987-06-01), Grosch et al.
patent: 4861709 (1989-08-01), Ulitzur et al.
Watkins et al., Appl. Environ. Microbiol., 54:1874-1875 (1988).
Ley et al., Can. J. Microbiol., 34:690-693 (1987).
Sadler et al., J. Am. Chem. Soc., 78:1251-1255 (1955).
Sales Brochure of Access Medical Systems, Inc., Cat. #IS-001 1/88 for Colilert MPN.
"Studies in Enzyme Cytochemistry III. Relationships Between Solubility, Molecular Association and Structure in Indigoid Dyes", Holt et al, Proc. Royal Soc. London, vol. 148B, pp. 495-505 (1958).
"Membrane Filter Method for Enumerating Escherichida coli", Dufour et al, Applied and Environmental Microbiology, vol. 41, No. 5, pp. 1152-1158 (May 1981).
"Membrane Filter Method for the Enumeration of Chlorine Damaged Coliforns in Drinking Water", Duncanson et al, Microbiology Dept., Univ. of Rhode Island.
Ferguson Wilfred J.
Roth Jonathan N.
Knode Marian
RCR Scientific, Inc.
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