Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1978-08-04
1980-07-29
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 26, G01N 3114
Patent
active
042151970
ABSTRACT:
Test means, such as a composition or a test device incorporating the composition, method of making the test device and process for determination of creatinine using the test means are disclosed. More particularly, creatinine is measured by incubating with a test means, such as a composition, comprising a creatinine hydrolyzing enzyme, creatine amidinohydrolase, sarcosine dehydrogenase and a tetrazolium indicator and observing any resultant color change. A colored formazan is produced in proporation to the creatinine present. The sensitivity of the system is increased and the detection limit lowered by including formaldehyde dehydrogenase, diaphorase and nicotinamide adenine dinucleotide (NAD). Creatine or sarcosine are measured in this system by omitting the creatinine hydrolyzing enzyme or the creatinine hydrolyzing enzyme and the creatine amidinohydrolase, respectively, from the composition used in testing for creatinine. The enzymes, tetrazolium indicator and other components are compatible and may be combined in a single solution. The compositions are advantageously incorporated with a carrier, such as a matrix or tablet, to provide a test device.
REFERENCES:
patent: 3867259 (1975-02-01), Forgione
patent: 3907644 (1975-09-01), Mollering et al.
patent: 3912588 (1975-10-01), Mollering et al.
patent: 3929580 (1975-12-01), Forgione et al.
patent: 4012286 (1977-03-01), Sanderson et al.
Herron Charles J.
Kepplinger Esther M.
Miles Laboratories Inc.
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