Test kits and methods for evaluating sterilization cycles

Details Test kits and methods for evaluating sterilization cycles Test kits and methods for evaluating sterilization cycles

1992-12-09

1994-11-22

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 34, 435810, 422 31, 422 61, C12Q 122, G01N 2100

Patent

active

053668723

ABSTRACT:
Methods and kits are provided for detecting viable bacteria or microorganisms at the completion of a sterilization cycle using rapid biological detection systems. The results of the detection systems can be read visually by non-technical personnel within one hour of the sterilization cycle at room temperature, resulting in a quick, cost-effective, and easy-to-use method or diagnostic kit.

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patent: 4803162 (1989-02-01), Smith et al.
patent: 5073488 (1991-12-01), Matner et al.
Vasley et al, Applied and Environmental Microbiology, vol. 58, No. 2, pp. 969-973, 1986.
(1986) A. Peter Snyder, et al., Applied and Environmental Microbiology, 51(5):969-973, "Pattern Recognition Analysis of In Vivo Enzyme-Substrate Fluorescence Velocities in Microorganism Detection and Identification".
(1992) Donald Vesley, et al., Applied and Environmental Microbiology, 58(2):717-719, "Fluorimetric Detection of a Bacillus stearothermophilus Spore-Bound Enzyme, .alpha.-D-Glucosidase, for Rapid Indication of Flash Sterilization Failure".
(1986) Propper Product Literature, "Duo-Spore, Biological indicators for monitoring effectiveness of sterilization procedures. For steam (gravity displacement of high vacuum), ethylene oxide gas or dry heat." (Long Island City, NY 11101.).
(1990) 3M Health Care Technical Report for Infection Control Products, "1291 Attest Rapid Readout Biological Indicator Technical Report." (St. Paul, MN 55144-1000.).
(1990) ATI, a Division of PyMaH Corp. Product Instructions, "ATI Disposable Biological Test Pack For Steam Sterilizers." (Somerville, NJ 08876.).

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