Test kit for the genetic detection of microorganisms

Details Test kit for the genetic detection of microorganisms Test kit for the genetic detection of microorganisms

1975-08-15

1977-07-26

Tanenholtz, Alvin E.

Textiles: fiber preparation

1951035M, C12K 110

Patent

active

040381439

ABSTRACT:
A test kit for detecting the presence of a specific microorganism in a biological sample utilizing the phenomenon of genetic transformation. The test kit comprises a transformable variant of said specific microorganism, such as auxotrophs which are distinguishable from their wild parent strain. The variants are transformed by deoxyribonucleic acid (DNA) extracted from microorganism cells present in a biological test sample. The detection of transformed cells indicates the presence of the specific microorganism under determination in the sample. The microorganism cells present in the biological sample are lysed utilizing an aqueous solution containing a surfactant capable of performing the lysing step.

REFERENCES:
patent: 3671400 (1972-06-01), Cekoric, Jr. et al.
Catlin et al., "Genetic Transformation of Neisseria Catarrhalis by Deoxyribonucleate Preparations Having Different Average Base Compositions," J. Gen. Microbiol. 37, (1964), pp. 341-352.

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