Test kit for determining the presence of organic materials and m

Details Test kit for determining the presence of organic materials and m Test kit for determining the presence of organic materials and m

1988-03-14

1993-08-31

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 56, 422 58, 435805, 435810, 436514, 436518, 436807, 436809, G01N 33543, G01N 33558

Patent

active

052408442

ABSTRACT:
A test card incorporates a colorimetric indicator test to determine the presence of a minute amount of a specific substance in a liquid medium. The test card includes top and bottom sheets adhesively secured to an intermediate frame member which, together with the top and bottom sheets, defines a filter chamber having a flat filter therein which incorporates a test portion. The test portion of the filter has a binding substrate to which antibodies of the specific substance have been bound. This binding substrate is in communication with a test port. In the method of the invention a substance to be tested is administered through the test port and contains an unknown amount of antigen. After the sample is administered an aqueous solution of enzyme labelled antigen is administered and, subsequently, a liquid substrate is added to cause a color change in the filter below the test port inversely proportional to the amount of antigen contained in the sample administered at the test port.

REFERENCES:
patent: 3791433 (1974-02-01), Moyer et al.
patent: 3811840 (1974-05-01), Bauer et al.
patent: 4366241 (1982-12-01), Tom et al.
patent: 4666863 (1987-05-01), Edwards et al.
Giegel et al, Clinical Chemistry, 28, 1894-1898, 1982.

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