Test kit for determination of organophosphate and carbamate pest

Details Test kit for determination of organophosphate and carbamate pest Test kit for determination of organophosphate and carbamate pest

1993-09-23

1994-12-20

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435 4, 435 32, 435975, 436 2, 436503, C12Q 100, C12Q 166, C12Q 118, G01N 3100

Patent

active

053745353

ABSTRACT:
A test kit and a rapid and sensitive method are provided for the multiple detection of organophosphate and carbamate pesticides. The test method employs an insect brain material that hydrolyzes a 6-substituted D luciferin ester, preferably a 6-substituted acetyl D luciferin ester, to produce D luciferin. Hydrolysis is inhibited by the pesticides in a test sample. Luciferase and adenosine triphosphate react with the D luciferin to produce oxyluciferin and emitted bioluminescence. The brain material is initially incubated with a test sample followed by the addition of the ester, luciferase and adenosine triphosphate. The kit contains the brain material, the ester and optionally luciferase and adenosine triphosphate, and may also contain means for incubating, and means for measuring emitted bioluminescence, and a control standard.

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