Test kit and method for the detection of microorganisms associat

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 79, 435 792, 435 794, 435961, 435975, 435259, G01N 33569

Patent

active

053345037

ABSTRACT:
An extraction composition is described which is buffered to a pH from about 8 to about 11 and which contains from about 1 to about 10 weight percent of a water-soluble cationic surfactant which is a quaternary ammonium salt or a mixture thereof and from about 1 to about 10 weight percent of an anionic surfactant which has a sulfate anion having from 6 to 14 carbon atoms and an alkali metal or ammonium cation. The extraction composition is used to extract antigens from Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis or Prevotella intermedia. Extracted antigens are determined using immunological methods. The extraction composition can be supplied as part of a diagnostic test kit.

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Microparticle Immunoassay Techniques, 2nd Ed., Seradyn, Inc., pp. 4-7 and 41-49 (1988).

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