Test kit and method for competitive specific binding assay

Details Test kit and method for competitive specific binding assay Test kit and method for competitive specific binding assay

1994-06-15

1996-12-31

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 793, 436518, G01N 3353, G01N 33543

Patent

active

055893449

ABSTRACT:
One or more specific binding ligands can be detected with a competitive immunoassay which utilizes a water-soluble conjugate of ligand and reporter enzyme for each target ligand. Target ligand is allowed to compete for a first receptor with the conjugate. Uncomplexed conjugate is then contacted with an immobilized second receptor to form a reaction product which can be used to generate a detectable signal. This assay provides a direct correlation of the generated signal to the one or more target ligands.

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Chemical Abstracts, vo. 110, No. 9, Feb. 7, 1989; B. Orsini et al. "A second antibody solid-phase enzyme immunoassay of prostaglandin E2 in human gastric juice" p. 66, No. 69 502x; & Clin. Chim. Acta 1988 178(3), 305-12, Abstract only.
Chemical Abstracts, Vo. 103, No. 15, Oct. 14, 1985; T. Tanaka et al. "Rat monoclonal antibody specific for prostaglandin E structure", p. 24, No. 116424r; & Biochem. Biophys. Acta 1985, 836(1), 125-33, Abstract only.

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