Test kit and method for amplification and detection of antigen c

Details Test kit and method for amplification and detection of antigen c Test kit and method for amplification and detection of antigen c

1994-08-18

1995-12-05

Spiegel, Carol A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 72, 435 75, 435 79, 435 791, 435 29, 435 34, 435839, 435834, 435848, 435874, 435881, 435883, 435 5, 435 6, 436527, 436531, 436813, G01N 3353

Patent

active

054728460

ABSTRACT:
A test kit and method for the amplification and detection of specific antigen cells using a probe. The method includes reacting the probe-specific cells with enzyme-conjugated molecules to form separate molecules. The specific antigen cells are mixed with a selected antibiotic which antibiotic is adversely affected by the enzyme in the reporter molecules and incubating the mixture to promote a bacterial chain reaction forming satellite colonies of bacteria microcolonies about the specific cells which amplifies the cells. The method then includes detecting the amplified probe-specific cells by observing the satellite colonies.

REFERENCES:
patent: Re31712 (1984-10-01), Giese
patent: 4002532 (1977-01-01), Weltman et al.
patent: 4478914 (1984-10-01), Giese
patent: 4656252 (1987-04-01), Giese
Premi, Thomas, MD and Battifora, Hector, MD. "Keratins Versus Epithelial Membrane Antigen in Tumor Diagnosis: An Immunohistochemical Comparison of Five Monoclonal Antibodies", Human Pathology, vol. 18, No. 7, Jul. 1987, pp. 728-734.

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