Test for the identification of glucose and for the determination

Details Test for the identification of glucose and for the determination Test for the identification of glucose and for the determination

1979-07-23

1981-07-28

Wyse, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 28, 435296, 422 56, C12Q 154

Patent

active

042810620

ABSTRACT:
Glucose identification and determination test. A test strip contains a metering zone, a reaction zone with glucose oxidase, and an indicator zone having at least one indicator signal zone of peroxidase and indicator applied in the form of stripes alternating with untreated intermediate spaces. The metering zone may be impregnated with a colorant, oxidizing, precipitating or buffering agent. The glucose oxidase is applied in an acid solution below the isoelectric point to prevent substrate inhibition by means of test fluid diffusion and may be in the form of an immobilized suspension. Together with a flow-inducing agent, the test strip allows a reaction not influenced by the time elapsed after sampling. Intermediate zones located between the reaction zone and the metering zone and/or the identification zone may be prepared with buffering substances. The test strip may be located within a capillary tube of 1-3 mm diameter and 40-120 length made of glass or plastic.

REFERENCES:
patent: 3359180 (1967-12-01), Evans et al.
patent: 3552925 (1971-01-01), Fetter
patent: 3811840 (1974-05-01), Bauer et al.
patent: 3964871 (1976-06-01), Hochstrasser
patent: 4046514 (1977-09-01), Johnston et al.

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