Test for quantitation of immunoglobulin and identification of ab

Details Test for quantitation of immunoglobulin and identification of ab Test for quantitation of immunoglobulin and identification of ab

1978-01-09

1979-08-07

Padgett, Benjamin R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 424 8, 424 12, G01N 3316, A61K 3900, A61K 4300

Patent

active

041637798

ABSTRACT:
A combined test for quantitating immunoglobulin and identifying the presence of abnormal homogeneous immunoglobulin usually of myeloma origin. In a direct test, a first fraction of serum sample is adsorbed to a solid surface and the adsorbed immunoglobulin is reacted with labeled antibody and the quantity of labeled antibody on the surface is measured. A second fraction of the serum sample is subjected to a competitive test in which normal immunoglobulin is adsorbed onto a second solid surface. Then, specific labeled antibody is competitively immunologically reacted with the immunoglobulin adsorbed on the surface and sample immunoglobulin. After washing, the labeled antibody on the second surface is measured to provide a quantitative measurement of sample immunoglobulin. Abnormal immunoglobulin is indicated if the ratio of immunoglobulin content of the sample as found by the competitive test to the quantity found by the direct test is 2:1 or greater.

REFERENCES:
patent: 4091089 (1978-05-01), Chichibo et al.

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