Test for pancreatic exocrine function

Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure

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128659, 424 15, 424 9, A61K 4900, A61K 4300, G01T 100

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active

042798868

ABSTRACT:
It has been determined that ingested vitamin B.sub.12 is bound by R protein in gastric juice, but that it is not capable of absorption in this form. In a system with a normally functioning exocrine pancreas, R protein is degraded by pancreatic proteases in the small intestine, which then allows the B.sub.12 to be transferred and bound to intrinsic factor protein (IF) from which it can be absorbed. This phenomenon serves as the basis for a test for pancreatic exocrine function in which the following compositions are ingested: (1) a complex of IF and B.sub.12 in which the B.sub.12 is labelled; (2) a combination of another binding material, such as R protein, and B.sub.12, in which the B.sub.12 is labelled (in preferred embodiments the B.sub.12 is labelled differently than the B.sub.12 in the IF-B.sub.12 complex); and (3) means for preventing R protein in the gastric juices from removing B.sub.12 from the IF-B.sub.12 complex, (in preferred embodiments such means are B.sub.12 analogues that bind to R protein in the gastric juices; but which do not bind to IF).

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Brugge et al., Gastroenterology, 78(1980), 937-949.
Allen et al., Gastroenterology, 75(1978), 761-762.
von der Lippe et al., Scand. J. Gastroenterol., 12(1977), 183-187.
Allen et al., J. Clin. Inv., 61(1978) 47-54, 1628-1634.
von der Lippe et al., Chem. Abstracts, vol. 88, No. 1, Jan. 1978, Abstract No. 451q.

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