Chemistry: physical processes – Physical processes – Crystallization
Patent
1976-07-07
1977-07-12
Yudkoff, Norman
Chemistry: physical processes
Physical processes
Crystallization
23259, 210359, 210500M, 210DIG23, 210DIG24, G01N 3122, G01N 3316
Patent
active
040351505
ABSTRACT:
A method and test of detecting occult blood in a stool or other physiologic fluid sample which offers novelty in that the reaction is carried out in the organic phase selected from the group consisting of methylene dichloride, petroleum ether, and dioxane in an aqueous/organic bi-phase system wherein the sample of occult blood is added to the aqueous phase, the bi-phase system is mixed by emulsification and a standard color-forming reaction involving organically soluble hemoglobin (heme), peroxide, and dye is carried out in the organic phase. Optionally, a filter paper porous only to organic solvents may be introduced to initially separate the aqueous and organic phases and further a device for mixing and separating immiscible liquids having two chambers telescopable into one another may be utilized. The reaction involving hemoglobin is carried out in acetic acid to produce heme which is later solubilized by the organic solvent. An advantage over previous tests lies in the effectiveness of the present method even in the presence of normally occurring amounts of ascorbic acid which inhibit all aqueous phase methods. The present test will give positive results for occult blood in the presence of at least 20 mg/dl of ascorbic acid (Vitamin C) or, alternatively, where the patient has ingested levels of Vitamin C or other reducing agent at an input of above 6 gm/day orally. This latter factor is quite important in present-day clinical practice where a substantial percentage of the patients are ingesting Vitamin C.
REFERENCES:
patent: 3865548 (1975-02-01), Padarver
Ingham, "An Improved and Simplified Benzidine Test for Blood in Urine and Other Clinical Material," Biochemical Journal, vol. 26, (1932), pp. 1124-1126.
Henry, "Clinical Chemistry Principles and Technics," pp. 779-782, (1964).
Kohn et al., "Inhibition of the Benzidine Blood Test by Ascorbic Acid," J. Biol. Chem., vol. 124, pp. 163-168, (1938).
Stas, M. E., "Het Aantonen Van Bloed in Faeces," Ned. Tijdschr. Geneesk, vol. 97, pp. 1709-1710, (1953).
Dent, "Occult-Blood Detection in Faeces of Various Animal Species," Lab. Pract., 1973, 22(11), pp. 674-676. Anal. Abst., 210, vol. 27, No. 1, July 1974.
Ferris Thomas G.
Hollander Barry I.
Latker Norman J.
Roberts, Jr. John S.
The United States of America as represented by the Secretary of
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