Test for determining frost hardiness of conifer seedlings and pr

Details Test for determining frost hardiness of conifer seedlings and pr Test for determining frost hardiness of conifer seedlings and pr

1994-11-09

1997-11-11

Feisee, Lila

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 792, 435975, 5303871, 530350, 800200, 800DIG51, G01N 3353, C07K 1600, C07K 14415, A01H 700

Patent

active

056862490

ABSTRACT:
A method of determining frost hardiness of a conifer seedling, which comprises detecting an amount of a cold protein in tissue of said conifer, the cold protein being a protein of approximately 19 kD that increase significantly in amount in the conifer during fall season (autumnal) months and that imparts frost hardiness to the seedling, and then assessing frost hardiness based on said detected amount. In the case of Pinus lambertiana, the cold protein includes the N-terminal sequence: Val--Ser--Gly--Thr--Ser--Ser--Thr--Glu--Glu--Val--Val--Gln--Asn--Glu--Ala- the cold protein includes the N-terminal sequence: Val--Ser--Gly--Thr--Ser--Ser--Thr--Glu--Glu--Val--Val--Gln--Val--Glu--Ala- invention also relates to conjugates and antibodies used in the method.

REFERENCES:
Yacoob RK & Filion WG. (1987) Biochem. Cell Biol. 65: 112-119.
Ekramoddoullah; et al--"Changes in protein profile of . . . "--Cdn. Journal of Plant Pathology 15:259-264 1993.
Ekramoddoullah--"Analyses of Proteins of Western White Pine Needles"--pp. 102-108 3rd IUFRO Rusts of Pine Working Party Con. 1989.
Ekramoddoullah--"An innovative approach to the Determination . . . " The Faseb Journal, Vo. 6, Nos. 4&5, Mar. 1992.
Ekramoddoullah--"Analysis of needle proteins and N-terminal amino acid . . . "--Tree Physiology 12, 101-106 1993.
Ekramoddoullah--"Monoclonal antibodies and their application in forestry"--Meeting of IUFRO Working Party S2.07-09, Canada, 1990.

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