Test device for determining the presence of leukocyte cells, est

Details Test device for determining the presence of leukocyte cells, est Test device for determining the presence of leukocyte cells, est

1995-03-30

1996-04-30

Gitomer, Ralph J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 23, 422 56, 560 1, 562589, C12Q 144, C12Q 137, C07C 6974

Patent

active

055124500

ABSTRACT:
A test device for determining the presence of leukocyte cells, esterase or protease in a test sample is disclosed. The test device comprises a carrier matrix having a reagent composition incorporated therein, wherein the reagent composition comprises a lactate ester having the structure ##STR1## wherein A is an alcohol blocking group, and wherein B--O-- is a residue of compound B--OH and provides a detectable response when the lactate ester is hydrolyzed; and a buffer. In a preferred embodiment, the lactate ester has the structure ##STR2## wherein X is O, S or NR.sup.2, R is aryl or lower alkyl, R.sup.1 is hydrogen or lower alkyl, and R.sup.2 is hydrogen, lower alkyl or aryl.

REFERENCES:
patent: 4064236 (1977-12-01), Dorn
patent: 4299917 (1981-11-01), Berger
patent: 4637979 (1987-01-01), Skjold
patent: 4814271 (1989-03-01), Hugl

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