Test device and method of preparing same, assay kit and method f

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 71, 435 792, 435974, 435975, 436808, 436809, 422 56, 422 57, 422 58, 422 61, C01N 33569

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active

050396048

ABSTRACT:
A kit and method have been developed which can be used to simultaneously detect antibodies to two HTLV or HIV antigens. The kit includes a solid carrier material, such as a microtest plate, having immobilized thereon a mixture of first and second viral antigens. The antigens are from any of HTLV-I, HTLV-II, HIV-I and HIV-II provided, however, that HTLV-II antigen is mixed only with HTLV-I antigen. The kit also comprises labeled-antibodies which are reactive with both the first and second antibodies in a test sample. A biological sample, such as a blood sample, is assayed by contacting it with the immobilized antigens to form reaction products between the immobilized antigens and the antibodies in the sample, followed by contact with the labeled antibodies to form labeled reaction products which can be detected in a suitable manner. The kit and method are useful for rapid screening of biological fluids. Also disclosed herein are a test device having immobilized antigens thereon and a method of preparing that device.

REFERENCES:
patent: 4315907 (1982-02-01), Fridlender et al.
Lee et al., "Serological Cross-Reactivity Between Envelope Gene Products of Type I and II Human T-Cell Leukemia Virus", Proc. Natl. Acad. Sci., U.S.A., 81(1984), 7579-7583.
Gazzolo et al., "Type-I and Type-III HTLV Antibodies in Hospitalized and Out-Patient Zairians", Int. J. Cancer, 36(1985), 373-378.
Clavel et al., "Molecular Cloning and Polymorphism of the Human Immunodeficiency Virus Type 2", Nature, 324(1986), 691-695.

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