Test composition, device and method for the colorimetric determi

Details Test composition, device and method for the colorimetric determi Test composition, device and method for the colorimetric determi

1997-06-05

1999-01-12

Soderquist, Arlen

Chemistry: analytical and immunological testing

Phosphorus containing

Of inorganic phosphorus compound in body fluid

436103, 436166, 436169, 422 50, 422 61, G01N 3300

Patent

active

058587977

ABSTRACT:
A unitized, one step test reagent composition, device and method is presented for the determination of phosphorus in various test sample fluids such as soil extracts. The invention is a modification of the Murphy and Riley methodology which utilizes a molybdate salt and a reducing agent to generate "molybdenum blue" in the presence of phosphate. In addition to the molybdate salt, the test reagent composition includes a chromogenic reducing agent which contributes to the color development and a solid buffer. The composition is preferably incorporated into a matrix such as paper for ease of handling.

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Y. Osawa et al. Bull. Chem. Soc. Jpn. 1991, 64, 2648-2655.
C. Matsubara et al. Analyst 1993, 118, 553-556.

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