Test carrier for analysis of a sample liquid, method of using a

Details Test carrier for analysis of a sample liquid, method of using a Test carrier for analysis of a sample liquid, method of using a

1989-04-13

1991-12-10

Nucker, Christine

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 58, 422 61, 422 681, 436524, 436531, 436535, 436536, G01N 33535

Patent

active

050717464

ABSTRACT:
The present invention provides a test carrier for the analytical determination of a component of a sample liquid with the help of immunological reagents having a conjugate layer (2, 12), which contains a soluble conjugate of a first immunological binding partner with a labelling, a porous fixing layer (3, 13), which contains a second binding partner in carrier-fixed form binding specifically with the first binding partner, and an optical detection layer (8, 18), wherein the fixing layer (3, 13) is constructed in such a manner that the suction speed with which the liquid is transported in it is so high that the time needed for the spreading out of the liquid in the fixing layer is considerably shorter than the incubation time which is needed for the completion of the specific binding reaction between the first binding partner and the second binding partner and the optical detection layer (8, 18) is so fixed on the test carrier (1, 11) that, in a first position, it is not in contact with the fixing layer (3, 13) making possible a liquid exchange but can be brought into a second position in which a liquid exchange between the fixing layer (3, 13) and the optical detection layer (8, 18) can take place.
The present invention also provides a process for the immunological analytical determination of a component of a sample liquid, as well as a process for the production of a fixing layer for a test carrier.

REFERENCES:
patent: 4740468 (1988-04-01), Weng et al.
patent: 4839297 (1989-06-01), Freitag et al.
patent: 4891313 (1990-01-01), Berger et al.

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