Test articles for performing dry reagent prothrombin time assays

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor

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435212, 422 56, 530381, 530384, C12Q 156, C12N 948, G01N 3122

Patent

active

054181410

ABSTRACT:
Test articles comprise a solid phase membrane having dry thromboplastin immobilized thereon or within. The thromboplastin is substantially free from substances which might cause aberrant functioning intermediate transition states as the thromboplastin is rehydrated with liquid sample. Coagulation neutral agents which facilitate rehydration of the dry thromboplastin are also provided on the solid phase membrane.

REFERENCES:
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patent: 4774192 (1988-09-01), Terminiello
patent: 5059525 (1991-10-01), Bartl
patent: 5110730 (1992-05-01), Edington et al.
patent: 5254350 (1993-10-01), Barrow
patent: 5270451 (1993-12-01), Hawkins
Tripodi A., Recombinant Tissue Factor as Substitute . . . Thrombosis & Haemostasis 67(1) 42-45 1992.
Hawkins P., Prothrombin Time Reagents Prepared . . . Clinical Hemostasis Review Apr. 1992.
Hawkins et al., "Prothrombin Time Reagents Prepared Using Recombinant Human Tissue Factor Produced in E. Coli", Clinical Hemostasis Review, Apr. 1992.
T. B. L. Kirkwood, "Calibration of Reference Thromboplastins and Standardisation of the Prothrombin Time Ratio", Thromb Haemostas 49(3):238-244 (1983).
S. Krishnaswamy, "The Interaction of Human Factor VIIa with Tissue Factor", J. Biol. Chem. 267(33):24696-24706 (1992).
Y. Nemerson, "Tissue Factor and Hemostasis", Blood 71(1):1-8 (1988).
J. D. Rawn, Biochemistry, Neil Patterson Publishers, pp. 219-232, Burlington N.C. 1989.
Shigematsu et al., "Expression of Human Soluble Tissue Factor in Yeast and Enzymatic Properties of Its Complex with Fator VIIa", J. Biol. Chem. 267(30):21329-21337 (1992).
Tripodi et al., "Recombinant Tissue Factor as Subsitute for Conventional Thromboplastin in the Prothrombin Time Test", Thrombosis and Haemostasis 67(1):42-45 (1992).

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