Chemistry: physical processes – Physical processes – Crystallization
Patent
1976-03-04
1979-01-09
Marantz, Sidney
Chemistry: physical processes
Physical processes
Crystallization
232306, 1951035R, 1951035A, 250365, 424 1, 424 7, 424 8, 424 12, 422104, G01N 3316, G01N 2100
Patent
active
041336399
ABSTRACT:
A body suitable for use in the quantitative detection of an unknown quantity of a biologically derived sample suitably by radioimmunoassay, fluorometric detection, or by spectrophotometry. The body includes a handle attached to a nonparticulate, nonswellable, impermeable continuous surface region. A diagnostic reagent (e.g., an antibody) is covalently attached to the surface region. The surface region is stirred by the handle in a solution of sample substance (e.g., antigen) for reaction with the diagnostic reagent. In a competitive binding technique, a labelled substance (e.g., fluorochrome labelled antigen) is simultaneously contacted with the sample. Thereafter, the surface region is washed, and the label is measured as by a fluorometer. The technique is also applicable to the so-called "sandwich technique" in which the labelled substance (e.g., labelled antibody) is reacted with the sample substance after it has been reacted with the diagnostic reagent. The above type of surface facilitates simple and effective separation of bound from free labelled substance and allows thorough washing to cleanse the surface of residual free and non-specifically bound labelled substance.
REFERENCES:
patent: 3646346 (1972-02-01), Catt
patent: 3720760 (1973-03-01), Bennich
patent: 3826619 (1974-07-01), Bratu
patent: 3935074 (1976-01-01), Rubenstein
patent: 3973129 (1976-08-01), Blumberg
R. Axen et al., Nature, vol. 214, 1302-1304 (Jun. 24, 1967).
"Radioimmunoassay Methods", European Workshop, Sep. 15-17, 1970, Edinburgh, Kirkham et al., eds.
Envall et al., Biochim. Biophys. Acta., 251 (1971) 427-434.
"Solid Phase Radioimmunoassay of Human Growth Hormone", Catt et al., J. Lab. & Clin. Med., (1966) 100, 31c.
International Diagnostic Technology, Inc.
Marantz Sidney
LandOfFree
Test article including a covalently attached diagnostic reagent does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Test article including a covalently attached diagnostic reagent , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Test article including a covalently attached diagnostic reagent will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1786799