Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Reexamination Certificate
2007-06-19
2007-06-19
Spector, Lorraine (Department: 1647)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
C514S012200, C435S069500, C530S402000, C424S085100
Reexamination Certificate
active
10774149
ABSTRACT:
The growth hormone supergene family comprises greater than 20 structurally related cytokines and growth factors. A general method is provided for creating site-specific, biologically active conjugates of these proteins. The method involves adding cysteine residues to non-essential regions of the proteins or substituting cysteine residues for non-essential amino acids in the proteins using site-directed mutagenesis and then covalently coupling a cysteine-reactive polymer or other type of cysteine-reactive moiety to the proteins via the added cysteine residue. Disclosed herein are preferred sites for adding cysteine residues or introducing cysteine substitutions into the proteins, and the proteins and protein derivatives produced thereby.
REFERENCES:
patent: 4588585 (1986-05-01), Mark et al.
patent: 4810643 (1989-03-01), Souza
patent: 4904584 (1990-02-01), Shaw
patent: 5166322 (1992-11-01), Shaw et al.
patent: 5206344 (1993-04-01), Katre et al.
patent: 5208158 (1993-05-01), Bech et al.
patent: 5681720 (1997-10-01), Kuga et al.
patent: 5766897 (1998-06-01), Braxton
patent: 5824778 (1998-10-01), Ishikawa
patent: 5824784 (1998-10-01), Kinstler et al.
patent: 5849535 (1998-12-01), Cunningham et al.
patent: 6632426 (2003-10-01), Osslund
patent: 0 458 064 (1991-11-01), None
patent: WO 93/00109 (1993-07-01), None
patent: WO 95/11987 (1995-04-01), None
patent: WO 95/32003 (1995-11-01), None
patent: WO 96/31537 (1996-10-01), None
Zurawski et al. Embo Journal 12, 5113-5119.
Bazan JF Science 257: 410-412.
Fujii I, Nagahara Y, Yamasaki M, Yokoo Y, Itoh S, Hirayama N. FEBS Lett. 410: 131-5.
Shanafelt AB, Kastelein RA. Proc Natl Acad Sci U S A. 86:4872-6.
Kuga T, Komatsu Y, Yamasaki M, Sekine S, Miyaji H, Nishi T, Sato M, Yokoo Y, Asano M, Okabe M, et al. Biochem Biophys Res Commun. 159:103-11.
Bazan,Immunology Today, 11:350-354 (1990).
Campbell et al.,J. Peptide Res., 49:527-537 (1997).
Cunningham et al.,Science, 243:1330-1336 (1989).
Goodson and Katre,Bio/Technology, 8:343-346 (1990).
Hill et al.,Proc. Natl. Acad. Sci. USA, 90:5167-5171 (1993).
Karpusas et al.,Proc. Natl. Acad. Sci. USA, 94:11813-11818 (1997).
Lowman and Wells,J. Mol. Biol., 234:564-578 (1993).
Mott and Compbell,Curr Opin Struct Biol, 6:114-121 (1995).
Sprang and Bazan,Curr. Op. Struct. Biol., 3:815-827 (1993).
Wells,Ann. Rev. Biochem., 65:609-634 (1996).
Zalipsky, S.,Adv. Drug Delivery Rev., 16:157-182 (1995).
Tanaka, H., Satake-Ishikawa, R., Ishikawa, M., Matsuki, S. and Asano, K. (1991) Cancer Research 51:3710-3714.
Bolder Biotechnology Inc.
Sheridan & Ross P.C.
Spector Lorraine
Stoica Elly-Gerald
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