Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-09-18
1994-09-13
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 691, 435 697, 435 698, 435 18, C12Q 168, G01N 3353, C12P 2100
Patent
active
053468120
ABSTRACT:
A method of screening a test substance for potential teratogenicity without employing vertebrate animals. In a representative embodiment, Drosophila primary embryonic cells, containing a hybrid game including a coding sequence for a detectable expression product under the control of a promoter sequence selected from among the 23 and 22 kDa Drosophila heat shock protein promoter sequences, are exposed to the test substance. The cells are thereafter assayed for the detectable expression product, the presence of the expression product in the cells providing an indication of the potential teratogenicity of the test substance. The primary embryonic cells containing the hybrid gene are conveniently provided by transformed flies.
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Ananthan-Nair Jayakumar
Voellmy Richard W.
Parr Margaret
The University of Miami
Zitomer Stephanie W.
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