Chemistry: analytical and immunological testing – Involving diffusion or migration of antigen or antibody – Through a gel
Patent
1985-02-25
1987-12-15
Marantz, Sidney
Chemistry: analytical and immunological testing
Involving diffusion or migration of antigen or antibody
Through a gel
422104, 436518, 436530, 436548, 436809, B01L 900, G01N 33543, G01N 33548, G01N 33559
Patent
active
047133490
ABSTRACT:
A templet (10) is provided having a lower plate (11) which receives a carrier sheet having antigens immobilized thereon as transferred from an electrophoresis gel. An upper plate (12) having a plurality of parallel channels (22) formed therein, is placed over the lower plate such that the channels lie over the carrier sheet. The two plates (11, 12) are then pressed tightly together, as by engagement with screws (16) at the periphery of the plates. A bridge bar (28) extends over the central portion of the plate and pressure screws (30) are mounted to the bridge bar in position to engage and apply pressure to the top surface of the upper plate (12). Various antibody containing liquids are introduced into the various channels through holes (25) extending to the ends of the channels; the solutions are allowed to remain in contact with the antigens on the carrier sheet for a period of time, after which the liquid is withdrawn from the channels, and the plates separated. The entire carrier sheet is subsequently exposed to tracer antibodies labeled radioactively or otherwise. The carrier sheet so treated shows a series of parallel vertical stripes, in each of which those horizontal antigen bands reactive with the particular antibody applied to the stripe are indicated. An antigen, or mixture of antigens, may thus be screened with many different antibodies simultaneously, and the user may compare the relative positions of antibody-labeled antigen bands on a single carrier sheet.
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Marantz Sidney
Saunders David A.
Wisconsin Alumni Research Foundation
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