Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering
Patent
1980-06-30
1981-10-13
Prescott, Arthur C.
Chemistry: electrical and wave energy
Apparatus
Coating, forming or etching by sputtering
204180G, 204180S, G01N 2728
Patent
active
042946840
ABSTRACT:
A template and method for using the same to form multiple concentration running gel tracks within a single electrophoretic gel bed. A template body comprises a support frame maintaining in parallel alignment a set of elongate bars. A sample well forming comb is provided for use in conjunction with the template body, said comb comprising a set of teeth projecting from a base and aligned in the same fashion as the elongate bars. The template body and comb are positioned in a gel bed with the well comb forming the electrophoretic origin and the bars forming tracts running parallel with the eventual current. A liquified gel is poured into the gel bed being excluded by the template in part, thereby forming the eventual gel running tracts and sample wells. After the gel hardens the template body is removed and the gel running tracks are filled with varying concentrations of gels. After the running gels have hardened the sample well forming comb is removed to allow introduction of a sample for subsquent electrophoresis.
REFERENCES:
patent: 2147458 (1939-02-01), Rohland
patent: 3495446 (1970-02-01), Williamson
patent: 3616387 (1971-10-01), Siebert et al.
patent: 3888759 (1975-06-01), Elson et al.
patent: 4061561 (1977-12-01), Fletcher et al.
patent: 4136007 (1979-01-01), Fujimori
patent: 4199428 (1980-04-01), Hayashi et al.
Serner et al., "Electrophoresis of Bacteriophage T7 and T7 Capsids in Agarose Gels", 28 Jrnl. of Virology, 917-928, (1978), Focus, vol. 1, No. 4, May/Jun. 1978.
Fangman, "Separation of Very Large DNA Molecules by Gel Electrophoresis," 5 Nucleic Acids Research, 653-665, (1978).
Johnson et al., "Electrophoresis of DNA in Agarose Gels," 16 Biochemistry, 4217-4224, (1977).
Ghosh et al., "Agarose Gel Electrophoresis of Serum Lipoproteins," 50 Anal. Biochem., 592-602, (1972).
Board of Reagents, University of Texas
Prescott Arthur C.
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