Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-05-08
1997-11-11
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 435 9151, 435 9152, 536 243, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104, C07H 2102
Patent
active
056862431
ABSTRACT:
A method for the detection of a target polynucleotide sequence wherein the target polynucleotide sequence is used as a template to direct hybridization of first and second polynucleotide probes to contiguous portions of the target. The hybridized probes are then ligated, preferably by covalent linkage of photoreactive functional groups present on each of the probes, to produce a ligated reaction product. The ligated reaction product is then multiplied with a polymerase such as Q-beta replicase to produce an enzyme reaction product which is then detected. The presence of the enzyme reaction product is indicative of the presence of the target polynucleotide sequence.
REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4786600 (1988-11-01), Kramer et al.
patent: 5449602 (1995-09-01), Royer et al.
Acevedo et al., Nature 321(6072):790-792, 1986.
Cruickshank Kenneth A.
Morrison Larry E.
Royer Garfield P.
Jones W. Gary
Murray William E.
The Regents of the University of California
Tran Paul B.
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