Template-dependent nucleic acid polymerization using...

Details Template-dependent nucleic acid polymerization using... Template-dependent nucleic acid polymerization using...

2006-06-13

2006-06-13

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

Reexamination Certificate

active

07060440

ABSTRACT:
A novel use of a template-dependent polymerase. The novel use is effected by employing the template-dependent polymerase for incorporating at least one oligonucleotide triphosphate onto a nascent oligonucleotide-3″-OH in a template pendent manner.

REFERENCES:
patent: 5436143 (1995-07-01), Hyman
patent: WO 98/23733 (1998-06-01), None
Moroney et al. Abortive Products as Initiating nucleotides during trnascription by T7 RNA polymerase. Biochemistry. vol. 30, No. 42, pp. 10343-10349, 1991.
Kayushin, et al, A Convenient approach to the synthesis of trinucleotide phosphoramidites-synthons for the generation of oligonucleotide/peptide libraries, Nucleic Acids Research, vol. 24, No. 19, 1996, pp. 3748-3755.
Lutz et al, An in vitro screening technique for DNA polymerases that can incorporate modified nucleotides. Pseudothymidine as a substrate for thermostable polymerases, Nucleic Acids Research, vol. 27, No. 13, 1999, pp. 2792-2798.
Moroney, et al, Abortive Products as Initiating Nucleotides during Transcription by T7 RNA Polymerase, Biochemistry, vol. 30, No. 42, 1991, pp. 10343-10349.
Schmidt et al, Information transfer from DNA to peptide nucleic acids by template-directed syntheses. Nucleic Acids Research, vol. 25, No. 23, (1997), pp. 4792-4796.

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